Analytical Sciences

Abstract − Analytical Sciences, 19(12), 1587 (2003).

Determination of Trace Procaine Hydrochloride by Differential Pulse Adsorptive Stripping Voltammetry with a Nafion Modified Glassy Carbon Electrode
Nianbing LI,*,*** Jianping DUAN,** and Guonan CHEN* 
*Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, P. R. China
**Analytical and Testing Center, Fuzhou University, Fuzhou, Fujian, 350002, P. R. China
***School of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing 400715, P. R. China
A sensitive and selective method for the determination of procaine hydrochloride with a Nafion-modified glassy carbon electrode has been developed. The voltammetric behavior of procaine hydrochloride on the Nafion-modified electrode indicated that the modified electrode not only increased the sensitivity of the determination of procaine hydrochloride, but also catalyzed the electrode process. Procaine hydrochloride was accumulated in Britton-Robinson buffer (pH 2.09) at a potential of -0.2 V (vs. SCE) for 180 s, and was then determined by differential pulse adsorptive stripping voltammetry. The effect of various parameters, such as the pH of the medium, the mass of drop-coated Nafion, the accumulation potential, the accumulation time and the scan rate, were investigated. Under the optimum conditions, a linear calibration graph was obtained in the concentration range of 6.0 x 10-8 to 6.0 x 10-6 mol l-1 with a correlation coefficient of 0.9987. The relative standard deviation was 4.18% for eight successive determinations of 1.0 x 10-7 mol l-1 procaine hydrochloride, and the detection limit (three times signal to noise) was 7.0 x 10-9 mol l-1. A study of interfering substances was also performed, and the method was applied to the direct determinations of procaine hydrochloride in the injection solution of procaine hydrochloride and in rabbit serum.