Analytical Sciences

A New Type of Chemically Bonded Phase for Reversed-Phase HPLC

Xiaojia HUANG, Junde WANG,  Xueliang LIU, and Runzi CONG

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan Road, Dalian, 116012, People's Republic of China

A new type of ether-bonded packing for reversed-phase HPLC (RP-HPLC) was synthesized by reacting 1-octanol with beta-(3,4-epoxycyclohexyl)ethyltrimethoxysilane, followed by coupling the product onto porous silica. The prepared packing was characterized by elemental analysis, solid-state ¹³C NMR, and Fourier transform infrared (FT-IR) spectroscopy. Chromatographic evaluations were performed by using a mixture of organic compounds as the analyte and methanol-water as binary mobile phase. The influence of the composition of organic modifier on the retention behavior of basic compounds was studied. The hydrolytic stability of the packing between pH 2.5 - 7.5 was also investigated. The results showed that the new stationary phase has excellent chromatographic properties and good hydrolytic stability.

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