Abstract − Analytical Sciences, 19(1), 49 (2003).
Development of the Single-Cell MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) Mass-Spectroscopic Assay
Masakazu SHIMIZU, Noriyuki OJIMA, Hitoshi OHNISHI, Tomoteru SHINGAKI, Yasuyuki HIRAKAWA, and Tsutomu MASUJIMA
Department of Analytical Life Science, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan
MALDI-TOF mass spectrometry was used to detect intracellular molecules from a single intact cell on monolayers of other cells. Intracellular molecules, e.g., histamine, were gradually increased in a mouse bone marrow-derived mast cell by a maturation process. A single cell was captured by a microsuction pipette, and the mass spectra of intracellular histamine were measured directly. Finally, the time course of the intracellular molecular contents and the maturation stage from a single cell were estimated.
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