Analytical Sciences

Nano-Kinetics of Probe-Particles in Solution Visualized by a Pin-Fiber Video Scope

Masashi SUZUTO,* Yasuyuki HIRAKAWA,* Hitoshi OHNISHI,* Shinji TACHINO,* Tomoteru SHINGAKI,* Edward M. EYRING,** and Tsutomu MASUJIMA* 

*Graduate School of Medical Science, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan
**Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA

The nano-kinetics of colloidal particles and living cells with the colloidal particles were visualized by a newly developed video scope. The system of the new video scope has a feature of fine controlling the illumination conditions by using a single optical fiber. This characteristic enables one to obtain clear images of living cells and the motions of colloidal particles by light-scattering effects. In the experiments, RBL-2H3 cells and gold colloidal particles were observed. Scattering images with high contrast and a dark background like in dark-field observations could be attained. In the experiments, a pulsed laser was also applied. The results obtained in this study could validate the effectiveness and possibility of a new video scope for applications to biological and biomedical fields.

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