Abstract − Analytical Sciences, 19(1), 151 (2003).
Determination of Trace Amounts of Bisphenol A in Urine by Negative-Ion Chemical-Ionization-Gas Chromatography/Mass Spectrometry
Tadashi TSUKIOKA,*1 John BROCK,*2 Sam GRAISER,*2 Johnny NGUYEN,*2 Hiroyuki NAKAZAWA,*3 and Tsunehisa MAKINO*4
*1 Nagano Research Institute for Health and Pollution, 1978 Komemura-Amori, Nagano 380-0944, Japan
*2 Centers for Disease Control and Prevention, 4770 Buford Highway NE Atlanta, GA, USA
*3 Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan
*4 Tokai University, Bouseidai, Isehara, Kanagawa 259-1193, Japan
*2 Centers for Disease Control and Prevention, 4770 Buford Highway NE Atlanta, GA, USA
*3 Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan
*4 Tokai University, Bouseidai, Isehara, Kanagawa 259-1193, Japan
We improved an analytical method for determining trace amounts of bisphenol A (BPA) in urine. BPA was subjected to enzymolysis and then to solid phase extraction with a C18 cartridge. The extract was eluted with methanol, and the eluate was concentrated under a nitrogen stream, and then pentafluorobenzylized in an alkali solution. The obtained pentafluorobenzylized compound was purifed using a florisil cartridge, followed by a determination using NCI-GC/MS. This method exhibited an excellent selectivity and reproducibility with a determination limit of 0.1 ng/ml.
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