Analytical Sciences

Integration of Chemical and Biochemical Analysis Systems into a Glass Microchip

Kiichi SATO,* Akihide HIBARA,* Manabu TOKESHI,** Hideaki HISAMOTO,* and Takehiko KITAMORI*^,** 

*Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan
**Integrated Chemistry Project, Kanagawa Academy of Science and Technology (KAST), 3-2-1 Sakado, Takatsu, Kawasaki 213-0012, Japan

This review focuses on the integration of chemical and biochemical analysis systems into glass microchips for generaluse. By combining multiphase laminar flow driven by pressure and micro unit operations, such as mixing, reaction, extraction and separation, continuous-flow chemical processing systems can be realized in the microchip format, while the application of electrophoresis-based chip technology is limited. The performances of several analysis systems were greatly improved by microchip integration because of some characteristics of microspace, i.e., a large specific interface area, a short molecular diffusion time, a small heat capacity and so on. By applying these concepts, several different analysis systems, i.e., wet analysis of cobalt ion, multi-ion sensor, immunoassay, and cellular analysis, were successfully integrated on a microchip. These microchip technologies are promising for meeting the future demands of highthroughput chemical processing.

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