Abstract − Analytical Sciences, 18(7), 757 (2002).
Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)2PIP(V) as a Nucleic Acid Probe
Gongwu SONG,* Ling LI,* Liming LIU,* Guangrong FANG,* Shaofang LU,** Zhike HE,** and Yun'e ZENG**
*Analysis and Testing Center Hubei University, Wuhan 430062, China
**College of Chemistry, Wuhan University, Wuhan 430072, China
**College of Chemistry, Wuhan University, Wuhan 430072, China
A new ligand, 2-phenyl(5-fluoro)imidazo[f]-1,10-phenanthroline (PIP(V)), and its coordination compounds, Ru(bpy)2PIP(V), were synthesized. The fluorescence spectrum of the interaction between Ru(bpy)2PIP(V) and DNA was studied, and a very strong fluorescence peak at a wavelength of 589 nm appeared. The optimum condition of analyzing DNA was decided. The method is simple, convenient and fast, and also has high sensitivity and good selectivity. It has been satisfactorily employed for determinations in synthesized samples.
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