Analytical Sciences

Effect of Di-2-ethylhexyl Sodium Sulfosuccinate Reversed Micelles on the Iron-Tetrasulfonatophthalocyanine-Catalyzed Fluorogenic Oxidation Reaction of L-Tyrosine with Hydrogen Peroxide

Ling WEI, Xiao-Lan CHEN, Huang-Hao YANG, and Jin-Gou XU 

The Key Laboratory of Analytical Science of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, P. R. China

The catalytic behavior of iron tetrasulfonatophthalocyanine (FeTSPc) for the oxidation reaction of L-tyrosine with H₂O₂ in a di-2-ethylhexyl sodium sulfosuccinate (AOT) reversed-micellar system (AOT/cyclohexane) was studied. It was indicated that the reversed micelles could not only enhance the catalytic activity of FeTSPc, but could also increase the fluorescence intensity of the product. Factors that may influence the catalytic reaction, including the concentration of AOT, the cosolubilized water, temperature and pH, were further examined. The possibility of its analytical application was also tested. Experimental results show that the calibration graphs for the determinations of FeTSPc and H₂O₂ under optimum conditions are linear over the range of 1.0 x 10^-8 - 1.0 x 10^-6 mol L^-1 and 0.0 - 3.0 x 10^-6 mol L^-1, respectively, with detection limits of 1.1 x 10^-9 mol L^-1 and 3.1 x 10^-9 mol L^-1 for FeTSPc and H₂O₂, respectively.

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