Abstract − Analytical Sciences, 18(4), 403 (2002).
A Novel Piezoelectric Biosensor forthe Detection of Phytohormone beta-Indole Acetic Acid
Jin LI,* Zhao-Yang WU,*Lang-Tao XIAO,** Guang-Ming ZENG,* Guo-He HUANG,*Guo-Li SHEN,* and Ru-Qin YU*
*State Key Laboratory of Chemo/Biosensingand Chemometrics, Chemistry and Chemical Engineering College, Department ofEnvironmental Science and Engineering, Hunan University, ChangSha 410082,P. R. China
**Key Laboratory of Phytohormones, Hunan AgriculturalUniversity, ChangSha, 410128, P. R. China
**Key Laboratory of Phytohormones, Hunan AgriculturalUniversity, ChangSha, 410128, P. R. China
A novel piezoelectric immunosensor has beendeveloped for the determination of beta-indole acetic acid (IAA) in dilutesolutions. The detection is based on competitive immunoreaction between ahapten (IAA) and an antigen (IAA-BSA, hapten-protein conjugation) bound toan anti-IAA antibody, immobilized on a quartz crystal microbalance (QCM).The frequency change (y) of the sensor caused by antigen is linearlyrelated to the logarithm of the concentration of IAA (x) in therange of 0.5 ng/ml - 5 ug/ml with a regression equation of the formy = - 23x + 151 (r = 0.9937).
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