Abstract − Analytical Sciences, 18(3), 283 (2002).
Simple and Sensitive Determination of Citrinin in Monascus by GC–Selected Ion Monitoring Mass Spectrometry
Pin-Yen SHU and Cheng-Huang LIN
Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Tingchow Road, Taipei, Taiwan
A new method for the qualitative and quantitative analysis of citrinin in Monascus by gas-chromatography-selected ion monitoring (SIM) mass spectrometry has been developed. GC separation of citrinin in Monascus extract was achieved without the need for chemical derivatization, and could be detected as a single peak when the SIM mode selected 5 prominent fragmentations (m/z of 220, 205, 177, 105 and 91). The quantitative detection limit for citrinin was ~1 ppb. Finally, the GC-separated analyte from Monascus extract, at a retention time of 10.89 min, was examined by the method of pattern recognition by comparison with a citrinin standard. The results show that the 2 compounds had a 94% similarity when the SIM mode was used.
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