Analytical Sciences

Application of a Surface Plasmon Resonance Sensor to Analyses of Amine Compounds with the Use of a Polymer Film and an Acid-base Reaction

Satoshi NISHIMURA,*  Toshifumi YOSHIDOME,** Tomoyuki TOKUDA,** Masaru MITSUSHIO,** and Morihide HIGO**

*Department of Applied Chemistry and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065, Japan
**Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065, Japan

A surface plasmon resonance (SPR) sensor was applied to analyses of some amine compounds (n-butylamine, isobutylamine, aniline, and N,N-dimethylaniline) by using a polymer film and an acid-base reaction in it. Poly(acrylamide) (PAA) was adopted as the polymer film and was immobilized on an Au film to prepare a sensor chip. Pivalic acid was entered into the PAA film as an acid. The PAA film with a thickness of 50 nm gave the highest sensitivity to the SPR sensor. Although water was better concerning the sensitivity for the SPR sensor as the solvent, ethanol was adopted because it dissolves well all of the amine compounds used. The Au film coated with the PAA film gave higher sensitivity for analyses of n-butylamine and isobutylamine, and lower sensitivity for analyses of aniline and N,N-dimethylaniline than an Au film without the PAA film. The PAA film containing pivalic acid gave 4 - 5 orders of magnitude higher sensitivity to the SPR sensor for analyses of all the amine compounds due to the reaction between pivalic acid and these amine compounds.

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