Analytical Sciences

Preparation and Characterization of Cyclodextrin Polymer and Its High-Performance Liquid-Chromatography Stationary Phase

Kwang-Pill LEE,*¹  Seong-Ho CHOI,*¹ Eun-Nyoung RYU,*¹ Jae Jeong RYOO,*¹ Jung Hag PARK,*² Yongseong KIM,*³ and Myung Ho HYUN*⁴

*1 Department of Chemistry Graduate School, Kyungpook National University, Taegu 702-701, Korea
*2 Department of Chemistry, Youngnam University, Taegu 712-749, Korea
*3 Division of Chemistry and Chemical Engineering, Kyungnam University, Masan 631-701, Korea
*4 Department of Chemistry, Pusan National University, Pusan 609-735, Korea

Cyclodextrin (CD) polymers were synthesized from the reaction of native CDs with a hexamethylene diisocyanate (HDI) compound in a dried DMF solution. The obtained CD polymer contained a range of 8 - 14% N due to HDI by elemental analysis. The physical and chemical properties of the CD polymers were characterized by IR, solid state ¹³C NMR, TGA, and DSC, respectively. An HPLC column was prepared using the CD polymer with a carbamate linker by a slurry method. Separation of the phenol isomers was conducted using the CD polymer stationary phase and CD by HPLC. Furthermore, an inclusion complex of the phenol isomer was studied by FT-Raman spectroscopy. From the HPLC and FT-Raman results, inclusion phenomena of o-, m-, and p-nitrophenol onto CD and CD polymers were analyzed.

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