Analytical Sciences

Glucose Sensing Based on Interdigitated Array Microelectrode

Peng JIN,* Akira YAMAGUCHI,** Fumika ASARI OI,*** Shigeki MATSUO,*** Jiubin TAN,* and Hiroaki MISAWA***

*Department of Automation Measurement and Control Engineering, Harbin Institute of Technology, No.92 West Dazhi Street, Harbin 150001, P. R. China
**Satellite Venture Business Laboratory of Photonic Nano-Materials, The University of Tokushima, 2-1 Minamijyosanjima, Tokushima 770-8506, Japan
***Department of Ecosystem Engineering, Graduate School of Engineering, The University of Tokushima, 2-1 Minamijyosanjima, Tokushima 770-8506, Japan

A micro glucose sensor consisting of an interdigitated array gold microelectrode was developed. The interdigitated array structure, which has 10 um band width and 10 um band gap, was fabricated in a small region (2.5 x5 mm²) on a quartz substrate. Glucose oxidase was chemically fixed onto the electrode surface through self-assembled monolayer of 11-mercaptoundecanoic acid; ferroceneacetic acid was used as electron mediator. Electrochemical properties of the glucose oxidase-immobilized microelectrode were investigated by cyclic voltammogram measurements. Results confirmed that the reductive ferroceneacetic acid generated at counter electrode diffuses through a narrow band gap (10 um) and can reach the working electrode surface.

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