Abstract − Analytical Sciences, 17(5), 649 (2001).
High-Sensitivity Analysis of Cyanide by Capillary Electrophoresis with Fluorescence Detection
Satoshi CHINAKA,* Seishi TANAKA,* Nariaki TAKAYAMA,* Naoko TSUJI,** Shinya TAKOU,*** and Kazumasa UEDA**
*Forensic Science Laboratory, Ishikawa Prefectural Police Headquarters, 2-1-1 Hirosaka, Kanazawa 920-8553, Japan
**Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University, 2-40-20 Kodatsuno, Kanazawa 920-8667, Japan
***Advanced and Fundamental Technology Department, Hamamatsu Industrial Research Institute, 1-3-3 Shin-Miyakoda, Hamamatsu 431-2103, Japan
**Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University, 2-40-20 Kodatsuno, Kanazawa 920-8667, Japan
***Advanced and Fundamental Technology Department, Hamamatsu Industrial Research Institute, 1-3-3 Shin-Miyakoda, Hamamatsu 431-2103, Japan
A capillary electrophoretic method for a high-sensitivity analysis of cyanide has been developed. Cyanide was derivatized with 2,3-naphthalenedialdehyde and taurine to give a fluorescent product of 1-cyanobenz[f]isoindole. This compound was detected with high sensitivity by fluorescence detection. The detection limit was 0.1 ng/mL, and the calibration curve was linear over the range 0.1 - 200 ng/mL. The precision of the migration time of within-run assays (n = 6) of 1 ng/mL cyanide standard solution was 0.14%. The precision of the peak area for the same runs was 1.0%. This method was applicable to blood analysis. Detection of the cyanide derivative by UV was also examined.
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