Analytical Sciences

Contents and Uptake Rates of Mn, Fe, Co, Zn, and Se in Se-Deficient Rat Liver Cell Fractions

Ken-ichiro MATSUMOTO,*  Tetsuya INAGAKI,* Rieko HIRUNUMA,** Shuichi ENOMOTO,** and Kazutoyo ENDO*

*Department of Physical Chemistry, Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan
**Radioisotope Technology Division, Cyclotron Center, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

The contents of manganese (Mn), iron (Fe), cobalt (Co), zinc (Zn), and selenium (Se) in nuclear (NU), mitochondrial (MT), microsomal (MC), and cytosolic (CS) fractions of liver homogenates of normal and selenium-deficient (SeD) rats were determined by instrumental neutron activation analysis (INAA). The uptake rates of these elements in the liver cell fractions of both groups of rats were determined by multitracer analysis (MTA). The results indicated that Se-deficiency caused a significant increase in the content of Fe in the MC fractions. The MTA showed that the uptake rate of Fe was highest in the MC fraction, and that the uptake rate in the fraction was similar between the SeD and normal rats.

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