Analytical Sciences

Determination of Hydrogen Peroxide by Iron(III) Complex of Thiacalix[4]-arenetetrasulfonate on a Modified Ion-Exchanger with Peroxidase-like Catalytic Activity¹

Junichi ODO,*  Yu INOMATA,* Haruhiko TAKEYA,** Setsuko MIYANARI,** and Hitoshi KUMAGAI**

*Department of Biological Chemistry, Faculty of Science, Okayama University of Science, Ridai-cho, Okayama 700-0005, Japan
**Chemical Technology Laboratory, Cosmo Research Institute, Satte, Saitama 340-0193, Japan

An iron(III) complex of thiacalix[4]arenetetrasulfonate on a modified anion-exchanger (Fe³⁺ -TCAS_A-500) has shown high peroxidase-like activity at pH 5 - 6 for the reaction of quinoid-dye formation between 3-methyl-2-benzothiazolinone hydrazone and N-(3-sulfopropyl)aniline in the presence of hydrogen peroxide. Utilizing the peroxidase-like activity of Fe³⁺ -TCAS_A-500 for this reaction, a method using Fe³⁺ -TCAS_A-500 was applied for the spectrophotometric determination of hydrogen peroxide. The calibration curve by the method using Fe³⁺ -TCAS_A-500 was linear over the range from 1 to 10 ug of hydrogen peroxide in a 1 ml sample solution. The apparent molar absorptivity for hydrogen peroxide was 2.4 x 10⁴ l mol^-1 cm^-1, which was about 80% of that by peroxidase under the same conditions. This determination method of hydrogen peroxide using Fe³⁺ -TCAS_A-500 was applied for the determination of glucose in diluted normal and abnormal control serum I and II.

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