Analytical Sciences

Abstract − Analytical Sciences, 17(12), 1421 (2001).

Determination of Trace Amounts of Boron in Steel by Reversed-phase High-Performance Liquid Chromatography with Azomethine-H as a Precolumn Derivatizaion Agent
Nobuo UEHARA,  Kazuhiro YAMAGUCHI, and Tokuo SHIMIZU
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, Yoto, Utsunomiya 321-8585, Japan
The determination of trace amounts of boron in steel by reversed-phase high-performance liquid chromatography (HPLC) is described. As a derivatizing reagent for the HPLC determination of boron, 8-hydroxy-1-(salicylideneamino)-3,6-naphtalenedisulfonic acid (azomethine-H) was used with a spectrophotometric detection. A peak of boron-azomethine-H chelate was resolved from other peaks using an acetonitrile-water (29 + 71 m/m) eluent containing 8 x 10-3 mol kg-1 tetrabutylammonium bromide and 5 x 10-3 mol kg-1 acetate buffer (pH 5.0). The lower determination limit (10 sigma) of boron was 3.3 x 10-8 mol dm-3 for a solution injected into HPLC, which is translated to 0.09 ugB/g when 0.1 g of a steel sample was subjected to the analysis. The analytical results of certified steel samples were in good agreement with the guaranteed values. The addition of ethylenediamine-N,N,N',N'-tetraacetate as a masking agent for the iron(III) matrix with the optimized eluent enables one to achieve the direct determination of trace amounts of boron in such steel sample solutions without any tedious matrix removal or preconcentration.