Analytical Sciences

Amperometric Biosensors for Sensing of Hydrogen Peroxide Based on Electron Transfer between Horseradish Peroxidase and Ferrocene as a Mediator

Yasuo NAKABAYASHI*^,** and Hajime YOSHIKAWA*

*Faculty of Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan
**HRC, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan

A non-conducting polymer film of 3-aminophenol, in which horseradish peroxidase was immobilized, has been prepared on a carbon paste electrode containing both ferrocene as a mediator and horseradish peroxidase by the electrochemical polymerization. The response currents of this hydrogen peroxide sensor linearly increased with increasing the concentration of hydrogen peroxide of 0.6 to 20 umol dm^-3; the straight line had a slope equal to 10.1 mA mol^-1 dm³ (r= 0.999). This hydrogen peroxide sensor may be available for the fabrication of other biosensors, e.g. glucose and cholesterol sensors, which are not influenced by easily oxidizable species, L-ascorbic acid and uric acid.

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