Analytical Sciences

Electric Resistance Variation of Packing Materials in CapillaryElectrochromatography by Using Fluorinated-BondedSilica and Octadecylsilane Phases

Chaiyavat CHAIYASUT,* Shinya KITAGAWA,** Hiroko WADA,* and Takao TSUDA*

*Department of Applied Chemistry, Nagoya Institute of Technology, Gokiso, Showa, Nagoya 466-8555, Japan
**Venture Business Laboratory, Nagoya Institute of Technology, Gokiso, Showa, Nagoya 466-8555, Japan

The linear relationship between electric resistance of the packed column for capillary electrochromatography and appliedvoltage was observed in several different effluents and packing materials (fluorinated-bonded silica and octadecylsilane).These linear relationships had different slope values which depended on the charges on the surface of the stationaryphase. If there are the negative charges on the stationary phase during the application of the electric field, the slope wasnegative value as observed in the fluorinated-bonded silica packed column. On the other hand, the slope was positivevalue in the fluorinated-bonded silica packed column using 0.1% tetrabutylammonium chloride as an effluent. Theinclination of the linear relationship between the electric resistance and applied voltage also depended on the kind of theeffluent, the concentration of effluent and the ratio of the organic modifier used. In the case of octadecylsilane packedcolumn, the electric resistance of the column did not depend on the applied voltage. The relationship between the electricresistance and the electroosmotic mobility was also studied.

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