Analytical Sciences

Application of Manganese-Tetrasulfonatophthalocyanine as aNew Mimetic Peroxidase in the Determination of Hydrogen Peroxide in Marine Surface Water Samples with p-Hydroxyphenylacetic Acid as a Substrate

Chang-Qing ZHU,* Dong-Hui LI,** Hong ZHENG,** Qing-Zhi ZHU,** Qiu-Ying CHEN,**and Jin-Gou XU**

*Department of Chemistry, Anhui Normal University, Wuhu 241000, P. R. China
**The Key Laboratory of Analytical Science of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, P. R. China

The dimerization of p-hydroxyphenylacetic acid by hydrogen peroxide in the presence of manganese-tetrasulfonatophthalocyanine(MnTSPc) has been applied to the determination of hydrogen peroxide in marine surface water samples. As MnTSPc was used as a substitute for horse radish peroxidase, the difficulties caused by possible loss of enzyme activity over time was eliminated. An EDTA-Mn(II) solution containing 2.0x10^-3 mol/l EDTA and 1.0x10^-6mol/l Mn(II) ion was used to reduce the interference caused by some metal ions and to increase the sensitivity. The detection limit of the method is 6.3x10^-8 mol/l. At a H₂O₂ concentration of 1.0x10^-6 mol/l, the standard deviation is 3.0%(n=6).

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