Analytical Sciences

Immunoassay Using Chemiluminescence Detection of Dyestuff-Containing Liposomes as a Labeling Reagent

Kazuhiko TSUKAGOSHI, Ž€Û©ideshi AKASAKA, Yasuo OKUMURA, Ryosuke FUKAYA, Miwa OTSUKA,Kazutoshi FUJIWARA, Hiroyuki UMEHARA, Reiko MAEDA, and Riichiro NAKAJIMA

Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan

A new immunoassay using chemiluminescence detection of dyestuff-containing liposomes as a labeling reagent isproposed. Human serum albumin labeled with Eosin Y containing-liposomes was employed as a labeling reagent for animmunoassay. After the immune reaction was carried out in the presence of an antibody-immobilized glass bead, thereactant solution was subjected to the capillary electrophoresis-chemiluminescence detection system. That is, thebound/free separation was conducted by use of glass beads, also, other coexisting compounds which might influencechemiluminescence detection were easily separated from the labeled human serum albumin by capillary electrophoresis.The labeled human serum albumin in the reactant solution was detected with high sensitivity. The amount of the labeledhuman serum albumin showed a good relationship to that of human serum albumin as an analyte through immunereaction. Human serum albumin could be determined over the range of 1x10^-6 - 5x10^-4 M. The present method was alsoapplicable to the determination of protein in a serum sample without being interfered with by coexisting constituents.

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