Analytical Sciences

Fluorescence Molecular Sensing for Endocrine-disrupting Chemicals and Their Analogues Based on Dansyl or Anthranilate Modified beta- and gamma-Cyclodextrins

Miyuki NARITA, Nobuaki OGAWA, and Fumio HAMADA *

Department of Materials-process Engineering and Applied Chemistry for Environments, Faculty of Engineering and Resource Science, Akita University, Tegata, Akita 010-8502, Japan

A fluorescent chemo-sensor for endocrine-disrupting chemicals has been studied by investigating fluorescence spectral changes with and without a guest molecule of regio-selective bis dansylglycine or anthranilate modified beta- and gamma-cyclodextrins. These host compounds show an increasing or decreasing pure monomer fluorescence intensity upon the addition of such guests as dioxin analogues, p-nonylphenol, 2,4-dichlorophenoxyacetic acid, bisphenol A, and diethyl phthalate. The extent of the fluorescence variations with the guest is used to display the sensing factors, deltaI/I₀, of these host molecules, where I and I₀ are the fluorescence intensities in the presence and absence of a guest and deltaI is I-I₀. These hosts exhibit various values of deltaI/I₀, which indicates the possibility of pattern recognition for endocrine-disrupting chemicals. These guests are detected with a remarkable response range by these hosts.

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