Analytical Sciences

Spectrophotometric Determination of Hydrogen Peroxide Using beta-CD-Hemin as a Mimetic Enzyme of Peroxidase

Yingping HUANG, Ruxiu CAI * , Luyuan MAO, Zhihong LIU and Houping HUANG

Department of Chemistry, Wuhan University, Wuhan, 430072, People's Republic of China

Various mimetic enzymes have been systematically studied concerning the color reaction for hydrogen peroxide oxidizing 4-aminoantipyrine (4-AAP) and N,N-diethylaniline (DEA) to yield a dye. The results have shown that beta-CD-hemin is the best mimetic enzyme for peroxidase among those tested, and that DEA is a quite good substrate for beta-CD-hemin. Meanwhile, the chromogenic reaction rate and sensitivity of 4-AAP-DEA-beta-CD-hemin system for determination of hydrogen peroxide were investigated. Zero to 8.4x10^-5 M hydrogen peroxide was determined with good accuracy and reproducibility. The molar-absorption coefficient of the chromogenic compound for hydrogen peroxide determined was found to be 1.65x10⁴ mol l^-1cm^-1. (Keywords: Mimetic enzyme, hydrogen peroxide determination, N,N-diethylaniline, spectrophotometry)

J-STAGE: View this article in J-STAGE