Abstract − Analytical Sciences, 15(9), 841 (1999).
Peroxidase-Like Activity of Ion Exchange Resins Modified with Metal-Porphine in Fluorescent Flow Injection Analysis
Akimasa IWADO*, Masaki MIFUNE** * , Takahiro HAZAWA**, Takashi MUKUNO*, Junko ODA**, Noriko MOTOHASHI*** and Yutaka SAITO**
*The Graduate School of National Science and Technology, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan
**Faculty of Pharmaceutical Science, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan
***Kobe Pharmaceutical University, Motoyamakita-machi, Higashinada, Kobe 653-8558, Japan
**Faculty of Pharmaceutical Science, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan
***Kobe Pharmaceutical University, Motoyamakita-machi, Higashinada, Kobe 653-8558, Japan
Peroxidase-like activity of anion-exchange resins modified with metal-tetrakis(sulfophenyl)porphine (M-TSPPr, M=Fe3+, Co3+ and Mn3+) were evaluated in the flow injection analysis of hydrogen peroxide using a fluorescent dimmer-formation reaction from chromogens, such as tyramine. A column (2.1 mmx50 mm) packed with Fe3+-TSPPr and a tube (0.8 mmx50 mm) packed with Mn3+-TSPPr accelerated the dimmer-formation reaction, indicating that they exhibit peroxidase- like activity just like the peroxidase-immobilized-column in the flow injection system. (Keywords: Peroxidase-like activity, metal-porphine, modified resin, flow injection analysis, fluorescence, hydrogen per-oxide, glucose)
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