Analytical Sciences

Determination of Trace Amounts of Arsenic(III) and Arsenic(V) in Drinking Water and Arsenic(III) Vapor in Air by Graphite-Furnace Atomic Absorption Spectrophotometry Using 2,3-Dimercaptopropane-1-sulfonate as a Complexing Agent

Chuan-Jier HSIEH, Chia-Hsin YEN and Mao-Sung KUO*

Department of Environmental Science, Tunghai University, Taichung 407, Taiwan, Republic of China

As(III) (12.5 - 200 ng) in drinking water (25 ml) reacted with 2,3-dimercaptopropane-1-sulfonate (DMPS, 0.60 mg) in ammonium acetate buffer (2.0 mmol, pH 5.5) and formed a complex of As(III)-DMPS. The complex was selectively retained on two Sep-Pak C18 cartridges in series, while As(V) could not be retained. Each cartridge was eluted with methanol (2.00 ml). After addition of Ni²⁺ (2.0 mg), a portion (20 ml) was introduced into a graphite cuvette and was atomized according to a temperature program. As(V) (up to 100 ng) in drinking water (25 ml) was pre-reduced to As(III) with L-cysteine. The concentration of As(V) was determined by subtracting As(III) from the final total concentration of As(III)+As(V). The method detection limits (3sigma) for As(III) and As(V) were 0.11 mg/l and 0.15 mg/l, respectively; the calibration graphs were linear up to 8.00 mg/l for As(III). Accuracies of 97.6 - 99.3% were obtained for As(III) and As(V) in drinking water with a precision within 4.0%. The proposed method could be applied to the determination of As(III) vapor in air with a detection limit of 2.8 ng. (Keywords: Arsenic(III), arsenic(V), drinking water, air, 2,3-dimercaptopropane-1-sulfonate, graphite-furnace atomic absorption spectrophotometry)

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