Abstract − Analytical Sciences, 15(2), 121 (1999).
Time-Resolved Fluorometric Detection of DNA Using a Tetradentate β-Diketonate Europium Chelate as a Label
Kenji YOSHIKAWA*1 , Jingli YUAN*1, *2 , Kazuko MATSUMOTO*1, *2* and Hiroko KIMURA*2, *3
*1 Department of Chemistry, Waseda University, Shinjuku, Tokyo 169-8555, Japan
*2 Japan Science and Technology Corporation (JST)
*3 Department of Forensic Medicine, Juntendo University School of Medicine, Bunkyo, Tokyo 113-8421, Japan
*2 Japan Science and Technology Corporation (JST)
*3 Department of Forensic Medicine, Juntendo University School of Medicine, Bunkyo, Tokyo 113-8421, Japan
A tetradentate β-diketonate europium chelate, 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl)-chloro-sulfo-o-ter conjugate for time-resolved fluorometric detection of lamdaDNA by hybridization with a biotinylated probe DNA in a microtiter well. The method gave a detection limit of 23 pg/well (solid-phase measurement) and 4 pg/well (solution-phase measurement) for specific DNA. (Keywords: Time-resolved fluorometric detection, lamdaDNA, europium fluorescent chelate)
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