Analytical Sciences

Effective Desorption of Humic Substances from Amberlite XAD-2 Resin with Sodium Dodecyl Sulfate

Masataka HIRAIDE and Kazutaka UCHITOMI

Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University,Chikusa, Nagoya 464-8603, Japan

The adsorption and desorption of humic and fulvic acids on a non-ionic macroreticular resin, Amberlite XAD-2, were studied from the view point of the concentration of aquatic humic substances. Microgram or milligram quantities of humic and fulvic acids were collected on a small column of pulverized XAD-2 (16 mm in diam.x5 mm height) and then desorbed with 10 cm³ of 0.1 mol dm^-3 sodium hydroxide solution containing 200 mg of sodium dodecyl sulfate (SDS). The resulting humic solution contained appreciable amounts of SDS (ca. 100 mg), which were removed by introducing the solution onto another XAD-2 column (7 mm in diam.x60 mm height, unpulverized resin). The SDS was completely retained on the resin because of the strong affinity, even in an alkaline solution, whereas nearly all humic substances were passed through the column. The validity of the proposed separation method was evaluated by using synthetic sample solutions. (Keywords: Humic acid, fulvic acid, Amberlite XAD-2, sodium dodecyl sulfate, water analysis)

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