Analytical Sciences

Separation Characteristics of Aminopropyl Silica GelsModified with Copper-Phthalocyanine as High Performance LiquidChromatography Stationary Phase

Masaki MIFUNE*1 , YoshihiroMORI*2 , MidoriONODA*2 , AkimasaIWADO*2 , NorikoMOTOHASHI*3 JunHAGINAKA*4 and YutakaSAITO*1

*1 Faculty of Pharmaceutical Sciences, Okayama University,Tsushima-Naka, Okayama 700-8530, Japan
*2 The Graduate School of Natural Science and Technology, OkayamaUniversity, Tsushima-Naka, Okayama 700-8530, Japan
*3 Kobe Pharmaceutical University, Motoyama-kita, Higashinada, Kobe658-8558, Japan
*4 Faculty of Pharmaceutical Sciences, Mukogawa Women's University,Kyubancho Koshien, Nishinomiya 663-8179, Japan

Separation characteristics of aminopropyl silica gelsmodified with copper-phthalocyanine (Cu-PCS_D) were investigatedin comparison with those of a pyrene stationary phase (PYE). By the use ofalkylbenzenes as samples, we have found that the pai-pai electroninteraction is dominant in the separation mode of a column packed withCu-PCS_D, while the hydrophobic interaction with alkyl groupsparticipates in the separation mode of a PYE column. The separation factor(alpha_n'+1) of a polyaromatic hydrocarbon (PAH) havingn'+1 aromatic rings by a PAH having n' aromatic rings islarger than that obtained from the PYE column. In particular, whenn' is 3, alpha_n'+1 is more than 6 times that ofthe PYE column in the maximum value. In addition, the planar recognitionability of the Cu-PCS_D column is much better than that of thePYE column. The above results indicate that the separation characteristicsof Cu-PCS_D are mainly due to the pai-pai electron interactionbased on Cu-PCS bound in parallel to the surface of the aminopropyl silicagel. (Keywords: Separation characteristics, copper-phthalocyanine-basedphase, modification, aminopropyl silica gel, pai-pai electron interaction,polyaromatic hydrocarbon)

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