Analytical Sciences

Simultaneous Catalyzing of Uricase- and Catalase-Like Reactions of Resins Modified with Metalloporphyrins and Its Application for Determination of Uric Acid 1

Junichi ODO* * and Hirotaka HASHIMOTO**

*Faculty of Science, Okayama University of Science, Ridai-cho, Okayama 700-0005, Japan
**Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Okayama 700-0082, Japan

Some anion-exchange resins modified with water-soluble metalloporphyrins exhibited an ability to catalyze simultaneously both uricase- and catalase-like reactions. The resin modified with manganese-tetrakis(4-sulfophenyl)porphine (Mn-TPPSr), which showed the highest activity among the resins tested, was useful for the indirect determination of uric acid by measuring the amount of formaldehyde produced. The calibration curve was linear over the range from 10 mg to 200 mg of uric acid with a relative standard deviation of 1.8% (n=10) for 200 mg uric acid. Mn-TPPSr was also applied for the determination of uric acid in sera. (Keywords: Uric acid, modified resin, catalytic activity, manganese-tetrakis(4-sulfophenyl)porphine, uricase, catalase)

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