Abstract − Analytical Sciences, 14(5), 913 (1998).
Single Molecule Detection by Laser Two-Photon Excited Fluorescence in a Capillary Flowing Cell
Joon Myong SONG*, Takanori INOUE*, Hirofumi KAWAZUMI** and Teiichiro OGAWA*
*Department of Molecular Science and Technology, Kyushu University, Kasuga-shi, Fukuoka 816-8580, Japan
**Department of Industrial Chemistry, Kinki University in Kyushu, Iizuka 820, Japan
**Department of Industrial Chemistry, Kinki University in Kyushu, Iizuka 820, Japan
The two-photon excited fluorescence of fluorescein and Rhodamine B in a flowing sample cell was observed using a self mode-locked Ti-sapphire laser (180 fs pulse). The detection limit of fluorescein was less than 2 molecules in the probe volume. The single molecule detection of Rhodamine was successfully performed in a flowing cell, and the number of photon bursts agreed approximately with that expected. (Keywords: Laser, two-photon fluorescence, single molecule detection, flow cell)
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