Abstract − Analytical Sciences, 14(4), 731 (1998).
Chemiluminescence Detection of Thiols by High-Performance Liquid Chromatography Using o-Phthalaldehyde and N-(4-Aminobutyl)-N-ethylisoluminol as Precolumn Derivatization Reagents
Akira SANO and Hiroshi NAKAMURA *
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Ichigaya-funagawara-machi, Shinjuku, Tokyo 162-0826, Japan
A method for the determination of thiols by high-performance liquid chromatography (HPLC) with chemiluminescence detection has been developed. The method is based on the precolumn derivatization of thiols with o-phthalaldehyde and (Keywords: Chemiluminescence detection, high-performance liquid chromatography, o-phthalaldehyde, N-(4-aminobutyl)-N-ethylisoluminol, glutathione, cysteine, human blood)
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