Analytical Sciences

Resolution of Amino Thiols in Time-Resolved Luminol Chemiluminescence Catalyzed by Peroxidases

Tamio KAMIDATE * , Toshiki TANI and Hiroto WATANABE

Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan

Hydrogen peroxide (H2O2) formed during the course of the Cu(II)-catalyzed oxidation of amino thiols with oxygen was detected by the Arthromyces ramosus peroxidase-catalyzed luminol chemiluminescence (CL) method. Two peaks appeared in the CL response curve in the catalytic oxidation of both cysteine (CySH) and gluthathione (GSH) and in that of both cysteamine and GSH. The resolution of two peaks corresponding to CySH and GSH was better than that corre-sponding to cysteamine and GSH. Only a strong CL flash appeared in the catalytic oxidation of both CySH and cys-teamine. The differences in the resolution of two peaks of amino thiols could be explained on the basis of the differences in the stabiliy constants between Cu(II) and amino thiols. (Keywords: Luminol, chemiluminescence, peroxidase, cysteine, cysteamine, gluthathione)

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