Analytical Sciences

Separation of Pollutant Phenols by Micellar Electrokinetic Chromatography with Double-Chain Surfactants Having Two Sulfonate Groups

Hiroya HARINO*, Shinji TSUNOI**, Junko YOSHIOKA**, Takashi ARAKI**, Araki MASUYAMA***, Yohji NAKATSUJI***, Isao IKEDA*** and Minoru TANAKA**

*Osaka City Institute of Public Health and Environmental Sciences, Tennoji, Osaka 543-0026, Japan
**Research Center for Environmental Preservation, Osaka University, Yamada-oka, Suita, Osaka 565-0871, Japan
***Department of Applied Chemistry, Faculty of Engineering, Osaka University, Yamada-oka, Suita, Osaka 565-0871, Japan

The separation of eleven priority (EPA) phenols and eighteen chlorophenols via micellar electrokinetic chromatography (MEKC) is described. Disodium 5,12-bis(dodecyloxymethyl)-4,7,10,13-tetraoxa-1,16-hexadecanedisulfonate (DBTHX), disodium 5,13-bis(dodecyloxymethyl)-4,7,11,14-tetraoxa-1,17-heptadecanedisulfonate (DBTHP) and disodium 5,13-bis(dodecyloxymethyl)-4,7,11,14-tetraoxa-9,9-dimethyl-1,17-heptadecanedisulfonate (DBTDMHP) were used as the double-chain surfactants. The EPA phenols were baseline separated by MEKC with 7.5 mM DBTHP. The detection lim-its of the phenols for a signal-to-noise ratio of 3 were in the range 0.5´±0 -2 - 3.0´±0 -2 mM. The relative standard devia-tion (RSD) of the capacity factors and peak areas for the phenols over the range of 0.31 - 1.8 mM were 0.21 - 1.4% and 1.9 - 4.3%, respectively. The separations of eighteen chlorophenols together with phenol were also carried out by MEKC with these surfactants. These phenols except for 2,3,5- and 2,4,5-trichlorophenol could each be separated with 10 mM DBTHX. (Keywords: Pollutant phenols, double-chain surfactants, micellar electrokinetic chromatography)

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